Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p078 | (1) | WCRB2014

The effect of cRNA concentration of artificial nuclease microinjected cytoplasmically to pronuclear porcine embryos on survival and development in vitro

Kamoshita Maki , Kato Tsubasa , Sagara Eri , Hisamatsu S , Sakaue M , Sakuma Tetsushi , Yamamoto Takashi , Ito Junya , Kashiwazaki Naomi

Although GH receptor (GHR) gene deletion causes a hereditary dwarfism and also shows a longer lifespan, the fact was shown only in mice. Because it was difficult to generate knockout (KO) animals other than mice. Recently, the generation of KO animals became possible without difficulty due to advances in genome editing technologies such as transcription activator-like effector nuclease (TALEN). In the present study, we examined the effect of cRNA concentration microinjected cy...